Um microscópio confocal que captura imagens com um campo de visão de 25 mm, até duas vezes a área dos scanners de ponto convencionais
A captura de imagens de amostras grandes, como tecidos, órgãos e organismos inteiros, requer a extensão tanto da área detectável quanto o aumento da velocidade de captura da imagem. O microscópio confocal A1 HD25 / A1R HD25 possui o maior campo de visão (25 mm) do mercado, permitindo que os usuários explorem além dos limites tradicionais da aquisição de imagens confocal.
Especificações
| A1 HD25 | A1R HD25 | |
|---|---|---|
| Scan head input/output port | 1 laser input port 2 signal output ports for standard, spectral and optional detector*1 |
|
| FOV | Ti2-E：Square inscribed in a Ø25 mm circle Ni-E/FN1：Square inscribed in a Ø18 mm circle |
|
| Image bit depth | 4096 gray intensity levels (12 bit) | |
| Recommended installation conditions | Temperature 23 ± 5 ℃, humidity 70 % (RH) or less (non-condensing) | |
| Z step | Ti2-E: 0.01 μm, 0.02 μm (with encoder control), FN1 stepping motor: 0.05 μm, Ni-E: 0.025 μm | |
| Compatible microscopes | ECLIPSE Ti2-E inverted microscope, ECLIPSE FN1 fixed stage microscope, ECLIPSE Ni-E upright microscope (focusing nosepiece type and focusing stage type) |
|
| Laser | A1 HD25 | A1R HD25 |
|---|---|---|
| LU-N3 3-laser unit | 405 nm, 488 nm, 561nm lasers are installed; built-in AOTF *Cannot be used with A1-DUS spectral detector |
|
| LU-N4/N4S 4-laser unit | 405 nm, 488 nm, 561 nm,640 nm lasers are installed; built-in AOTF *LU-N4 cannot be used with A1-DUS spectral detector |
|
| LU-NV series laser unit | Compatible lasers : 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm, 647 nm ; built-in AOTF | |
| Standard fluorescence detector | A1 HD25 | A1R HD25 |
|---|---|---|
| Wavelength | 400-750 nm | |
| Detector | A1-DU4-2 4 Detector Unit: 4 Multi-Alkali PMTs A1-DUG-2 GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 Multi-Alkali PMTs |
|
| Filter cube | 6 filter cubes commonly used for a microscope mountable on each of three filter wheels Recommended wavelengths: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 595/50, 700/75 |
|
| Diascopic detector (Option) | A1 HD25 | A1R HD25 |
|---|---|---|
| Wavelength | 485-650 nm | |
| Detector | Multi-Alkali PMT | |
| Scan head | A1 HD25 | A1R HD25 |
|---|---|---|
| Standard image acquisition | Scanner: galvano scanner x2 Pixel size: max. 4096 x 4096 pixels Scanning speed: Standard mode: 1.4 fps (512 x 512 pixels, bi-direction, 0.72x zoom), 2 fps (512 x 512 pixels, bi-direction, 1x zoom) Fast mode: 10 fps (512 x 512 pixels, bi-direction, 8x zoom), 200 fps (512 x 16 pixels, bi-direction, 8x zoom)*2 Zoom: 0.72-1000x continuously variable Scan mode: X-Y, X-T, X-Z, XY rotation, Free line, Line-Z |
|
| High-speed image acquisition | — | Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), galvano scanner (Y-axis) Pixel size: max. 1024 x 1024 pixels Scanning speed: 15 fps (1024 x 1024 pixels), 30 fps (512 x 512 pixels), 60 fps (256 x 256 pixels) to 720 fps (512 x 16 pixels), 7,800 lines/sec (line speed) Zoom:0.72x, 0.82x, 0.9x, 1x, 1.2x, 1.5x, 1.75x, 2x, 2.4x, 3x, 4x, 5x, 6x, 7x, 8x Scan mode: X-Y, X-T, X-Z Acquisition method: High-speed image acquisition |
| Dichroic mirror | Low-angle incidence method, Number of positions: 8 Standard filter: 405/488/561/640, BS20/80 Optional filter:405/488, 405/488/561, 405/488/543/640, 457/514 |
|
| Pinhole | 12-256 μm variable (1st image plane) | |
| Spectral detector (option) | A1 HD25 | A1R HD25 |
|---|---|---|
| A1-DUS spectral detector unit | Number of channels: 32 Wavelength detection range: 400 – 750 nm Spectral image acquisition speed: 4 fps (256 x 256 pixels) Maximum pixel size: 2048 x 2048 (Spectral mode/Virtual filter mode) Wavelength resolution: 2.5/6.0/10.0 nm, wavelength range variable in 0.25 nm steps Compatible with galvano scanner only |
|
| A1-DUVB-2 GaAsP detector unit | Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (A1-DUVB-OP) with a user-defined dichroic mirror and barrier filter Wavelength detection range: 400 – 720 nm, narrowest: 10 nm, broadest:320 nm Maximum pixel size: 4096 x 4096 (CB mode/VB mode) Wavelength resolution: 10 nm, wavelength range variable in 1 nm steps Compatible with galvano and resonant scanners |
|
| Option | A1 HD25 | A1R HD25 |
|---|---|---|
| Motorized XYZ | Motorized XY stage (for Ti2-E/Ni-E), High-speed Z stage (for Ti2-E), High-speed piezo objective-positioning system (for FN1/Ni-E) | |
| Photostimulation module*3 (for Ti2-E) |
XY galvano scanning unit (Light source: LU-N3/N4 Laser Unit) DMD module (Light source: C-LEDFI Epi-FL LED illuminator, LU-N3/N4 laser unit) Stimulation form: ROI/line/point Stimulation mode: Sequential, Simultaneous |
|
| Software | A1 HD25 | A1R HD25 |
|---|---|---|
| Acquisition/analysis | Basic software: NIS-Elements C Optional software for high-resolution acquisition: NIS-Elements C-ER |
|
| Display/image generation | 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing | |
| Image format | JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS | |
| Application | FRAP, FLIP, FRET(option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization | |
| Control computer | A1 HD25 | A1R HD25 |
|---|---|---|
| OS | Windows 10 Pro 64bit, English or Japanese version, OS Version 1709 Windows 7 Professional, 64bit, SP1 English or Japanese version, Windows Update KB3118401 or later |
|
| CPU | Intel Xeon W-2125 (4.0GHz, quad core, 8.25 MB, 2666 MHz) or higher | |
| RAM | 32GB or 64GB | |
| HDD | 1st HP Z Turbo G2 512GB PCIe M.2 SSD 2nd SATA HDD 2TB |
|
| Optical Drive | Super Multi drive, up to x16 speed or higher | |
| Graphics | NVIDIA Quadro P600 or higher (NIS-Elements C-ER: NVDIA Quadro P4000) (PCI Express/two-screen split display supported) |
|
| Extension slot | Two PCI Express 3.0 (x16) slots (one slot to be used for graphics) One PCI Express 3.0 (x8) slot Two PCI Express 2.0 (x4) slot |
|
| LAN port | 10/100/1000 Network/Interface x 2 (for connection to controller, for connection to external LAN) | |
| Monitor | 1600 x 1200 or higher resolution, dual monitor configuration recommended | |
*1 FCS/FCCS/FLIM is possible in combination with third-party systems
*2 Fast mode is compatible with zoom 8-1000x and scanning modes X-Y and X-T. It is not compatible with Rotation, Free line, CROP, ROI, Spectral imaging, Stimulation and FLIM.
*3 Adapter for Ti2-LAPP system, light source, hybrid dichroic mirror for photoactivation and imaging, and control board are all required.
Dimensions and weight
| Scan Head | 277(W) x 163(H) x 366(D) mm | Approx. 14.5 kg |
|---|---|---|
| Controller | 360(W) x 575(H) x 599(D) mm | Approx. 40 kg |
| A1-DU4-2 4 Detector Unit | 360(W) x 199(H) x 593.5(D) mm | Approx. 16 kg |
| A1-DUG-2 GaAsP Multi Detector Unit | 360(W) x 199(H) x 593.5(D) mm | Approx. 16 kg |
| A1-DUS Spectral Detector Unit | 360(W) x 323(H) x 593.5(D) mm | Approx. 26 kg |
| A1-DUVB-2 GaAsP Detector Unit | 360(W) x 114(H) x 595.5(D) mm | Approx. 10 kg |
| LU-N4/N4S/N3 Laser Unit | 360(W) x 210(H) x 593.5(D) mm | Approx. 19 kg |
| LU-NV Laser Unit | 400(W) x 781(H) x 685(D) mm | Approx. 70 kg |
| LU Controller Box B (for LU-NV) | 400(W) x 123(H) x 687(D) mm | Approx. 7 kg |
Power source
| Scan Head and Controller | Input 100-240V ± 10%, 50-60Hz, 5A-2A |
|---|---|
| Computer Unit | Input 100-240V ± 10%, 50-60Hz, 12A-10A |
| LU-N4/LU-N4S/LU-N3 | Input 100-240V ± 10%, 50-60Hz, 2A max. |
| LU-NV Series | Input 100-240V ± 10%, 50-60Hz, 4.8A max. |
| LU Controller Box B (for LU-NV) | Input 100-240V ± 10%, 50-60Hz, 1A max. |
| Inverted Microscope Ti2-E and HG Fiber Illuminator Intensilight | Input 100-240V ± 10%, 50-60Hz, 6.3A max. |
